Never Lose Your Sialic Acid Manufacturer Again
Previous work using spectrophotometric assays reported that E. coli YjhC showed a weak interaction with Neu5Ac, with an apparent Km of 68.8 mm (20). Here, we used ESI-MS to assess the activity of YjhC against 2,7-anhydro-Neu5Ac or Neu5Ac. The glycan array binding data provide independent support of AAV1 interaction with α2,3 and α2,6 trisaccharides. Our …